Optimization of ultrasonic-assisted extraction and antioxidant capacity of total flavonoids from lentinus edodes
The ultrasonic-assisted extraction of total flavonoids from lentinus edodes was optimized by single factor experiments and orthogonal experimental designs,and the antioxidant capacity of the total flavonoids extract was evaluated by scavenging hydroxyl free radical (·OH) and superoxide anion free radical (O2-·).The results showed that ethanol concentration significantly affected the extraction yield of total flavonoids.The optimum extraction condition was determined as following:ethanol concentration 60％,ratio of solid to liquid 1∶30 (g∶mL),extraction temperature 65 ℃,and extraction time 60 min.The yield of total flavonoids under these conditions was 0.802％± 0.018％.The total flavonoids extract obtained had certain scavenging capacity against ·OH and O2-·,and the scavenging capacity against ·OH was stronger than VC at the same concentration,but scavenging capacity against O2-· was weaker than that of VC.
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