Effects of mixed enzyme preparations on lycopene extraction and molecular structure
Lycopene is a fat-soluble carotene pigment which has antioxidant,scavenging capacities on oxygen radicals,preventing lipoprotein and DNA oxidization,and-aging,tumor-prevention and other healthy physiological effects.Extracting lycopene from tomato pomace by combined treatment with cellulose pectinase and ethyl acetate had been investigated.The results showed that pectinase played more important role in the process.The optimum conditions of extraction were as follows:0.2mL/g mixed enzyme and ethyl acetate as extract agent,hydrolysis time 3h at 35℃.Changes of molecular structure were revealed through C30-HPLC.The results indicated that some of the trans-isomer of lycopene changed into cis-isomer by mixed enzyme treatment,which can increase the absorption by human body and provide the scientific data for further develop of healthy food.