首页|混合酶处理对番茄红素提取工艺及结构的影响

混合酶处理对番茄红素提取工艺及结构的影响

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番茄红素是一种脂溶性的类胡萝卜素,具有很强的抗氧化活性,能够清除氧自由基,可防止脂蛋白和DNA氧化损伤,还具有抗衰老和降低肿瘤发生的作用,因此具有重要的研究价值.本文是以番茄皮渣为原料,乙酸乙酯为萃取剂,研究添加纤维素酶和果胶酶提取番茄红素的实验,分别考察了混和酶用量、酶解时间和酶解温度对番茄红素萃取效果的影响.结果表明,果胶酶对番茄红素的提取率起主要作用.最佳提取条件为A1B1C2,即混合酶用量为0.2mL/g,酶解时间为3h,酶解温度为35℃,得到的萃取液中番茄红素含量最高.C30-HPLC色谱图结果表明,经过酶处理的番茄红素有少部分反式异构体转变为顺式异构体,有助于番茄红素在人体内的吸收及协同效应,为番茄红素保健食品的开发提供试验依据.
Effects of mixed enzyme preparations on lycopene extraction and molecular structure
Lycopene is a fat-soluble carotene pigment which has antioxidant,scavenging capacities on oxygen radicals,preventing lipoprotein and DNA oxidization,and-aging,tumor-prevention and other healthy physiological effects.Extracting lycopene from tomato pomace by combined treatment with cellulose pectinase and ethyl acetate had been investigated.The results showed that pectinase played more important role in the process.The optimum conditions of extraction were as follows:0.2mL/g mixed enzyme and ethyl acetate as extract agent,hydrolysis time 3h at 35℃.Changes of molecular structure were revealed through C30-HPLC.The results indicated that some of the trans-isomer of lycopene changed into cis-isomer by mixed enzyme treatment,which can increase the absorption by human body and provide the scientific data for further develop of healthy food.

cellulosepectinasecis-isomertrans-isomer

张玉丹、高应权、刘蕊、杨阳、任航、刘沐霖

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新疆红帆生物科技有限公司,库尔勒841100

哈尔滨市食品药品检验检测中心,哈尔滨 150025

纤维素酶 果胶酶 顺式异构体 反式异构体

2016

中国食品添加剂
中国食品添加剂和配料协会

中国食品添加剂

CSTPCD北大核心
影响因子:0.677
ISSN:1006-2513
年,卷(期):2016.(1)
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