Studies on the effects of different polysaccharides extraction process from Auricularia auricula on antioxidant activities and α-glycosidase inhibitory
In order to evaluate the effects of different polysaccharides extraction process from the fruiting body of Auricularia auricular-judae on antioxidant activities and α-glycosidase inhibitory,extracts were prepared from Auricularia auricular-judae by water extraction,cellulose enzyme extraction and alkali solution extraction.The polysaccharides content in extracts and the effects of extracts on activity of α-glycosidase,hydroxyl radicals scavenging,chelating effects and Caenorhabditis elegans resistance to H2O2 were determined.The polysaccharides content in extracts from A.auricular-judae with different extraction process are different,and the highest content of 50.35％ ± 1.26％ was recorded for alkali solution extraction,and the lowest of 1.65％ ± 0.27％ was recorded for water extraction.The inhibition effect on α-glycosidase activity of the extract from the alkali solution extraction was similar to that of the enzyme extraction (39.24 ± 1.17％ and 39.61 ± 1.21％),and the inhibition effect of water extract was only 13.67 ± 0.97％.The highest hydroxyl radicals scavenging and chelating effects were recorded for the extract prepared from the enzyme extraction,and the effects were significant difference compared with the same concentration (2 mg/mL) of water extract and alkali extract (P ＜ 0.05).In addition,the extracts of enzyme and alkali extraction had significantly enhanced nematodes resistant to H2O2,and they could prolong the nematodes life in the oxidative stress state coincident with control group.
国家科技期刊平台
NSTL
万方数据
维普
