Study on the separation and purification of total flavonoids from black currant leaves by macroporous resins
Black currant leaves contain a variety of flavonoids,which has many functions,such as anti-tumor,anti-oxidation,anti-bacterial and anti-inflammatory.In this paper,six kinds of macroporous resins (AB-8,NKA-9,D101-1,X-5,D4006,D4020) were used to separate and purify flavonoids crude extract from the leaves of black currant.Based on the study of the static adsorption and desorption of macroporous resin,three kinds of macroporous resins (NKA-9,D4020,D101-1) were selected.Then the kinetics and adsorption isotherm for separation and purification were studied.The best conditions were determined as:temperature 25℃,NKA-9 macroporous resin,flavonoids concentration 0.9311 mg/mL,sample volume 60 mL,flow rate 1 mL/min;the eluent (absolute ethyl alcohol) dosage 100 mL,flow rate 1 mL/min.The flavonoids compound I was isolated by Sephadex LH-20 gel.The standard curve was obtained by high performance liquid chromatography and the purity was 86.37%.
NKA-9 macroporous resinseparation and purificationblack currant leavesflavonoid compounds