首页|AB-8大孔树脂纯化黑果枸杞原花青素及其稳定性研究

AB-8大孔树脂纯化黑果枸杞原花青素及其稳定性研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
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  • 维普
采用AB-8大孔树脂纯化黑果枸杞原花青素,并研究纯化后黑果枸杞原花青素的稳定性.通过静态与动态吸附、解吸实验,研究得出AB-8大孔树脂纯化黑果枸杞原花青素的最佳条件为:上样液浓度为1mg/mL,上样流速为2 mL/min,上样量为5 BV,洗脱剂浓度为70％,洗脱流速为2 mL/min,洗脱量为6 BV,在此条件下获得原花青素纯度为(62.36±0.76)％,回收率为(67.43±1.48)％;黑果枸杞原花青素对光和温度较敏感,在避光和低温条件下稳定性较好;氧化剂和还原剂对原花青素稳定性影响较大;Fe3+、Al3+、Zn2+和Cu2+均可降低原花青素的稳定性,Na+、K+、Mg2+和Ca2+对原花青素稳定性的影响不大;pH对原花青素的稳定性影响较大,当pH3 ～7时原花青素较稳定;食品添加剂:蔗糖、维生素C、柠檬酸、山梨酸钾对原花青素稳定性影响不明显.该结果为促进黑果枸杞原花青素的加工及利用提供一定的理论基础.
Purification of proanthocyanidins from Lycium ruthenicum Murr.by AB-8 macroporous resin and its stability
AB-8 macroporous resin was used to purify proanthocyanidins from Lycium ruthenicum Murr..The optimized purification parameters after static adsorption and desorption tests were:sample concentration 1mg/mL,eluent concentration70％,sample flow rate 2 mL/min,elution amount 5 BV,elution flow rate 2 mL/min,eluent amount 6 BV.Under these conditions,the purity of proanthocyanidins was (62.36 ± 0.76) ％,and the recovery rate was (67.43 ± 1.48) ％.Proanthocyanidins were sensitive to light and temperature,the stability was poor under strong light and high temperature.Oxidantive and reducing agent had significant effect on the stability;ions such as Fe3+,A13+,Zn2+,and Cu2+ can decrease the stability,while Na+,K+,Mg2+and Ca2+ had no significant effect;pH value had a great influence and sucrose,VC,citric acid and potassium sorbate had no significant effect on the stability.

lycium ruthenicum murr.proanthocyanidinsmacroporous resinpurificationstability

李三省、许丹丹、张富粟、张鑫、谷盼盼、王芳梅、许若男、王亮

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新疆昌吉产品质量检验所,昌吉831100

新疆大学生命科学与技术学院,乌鲁木齐830046

乌恰县托运乳业服务中心,乌恰县845450

黑果枸杞 原花青素 大孔树脂 纯化 稳定

自治区科技支疆项目

2017E0228

2018

中国食品添加剂
中国食品添加剂和配料协会

中国食品添加剂

CSTPCD
影响因子：0.677
ISSN：1006-2513
年,卷(期)：2018.(3)
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