Improvement effects and mechanism of potato resistant starch on insulin resistance in HepG2 cells
High-performance liquid chromatography-tandem mass spectrometry was used to determine the composition of short-chain fatty acids (SCFAs) produced from the digestion of potato resistant starch (resistant starch,RS). An insulin-resistance (IR) model in HepG2 cell was established through high glucose and high insulin induction. To explore the improvement effects and mechanisms of SCFAs derived from potato RS on IR HepG2 cells,different concentrations of SCFA (0.01,0.1,1 μmol/L) on glucose consumption,protein expression and mRNA content of AMPK,Akt,PI3K,GLUT 2 and GLUT 4 in IR HepG2 cells were assessed. The results showed that the ratio of acetic acid,propionic acid and butyric acid produced by potato RS digesting was 2︰4︰1. Glucose at 30 mmol/L and insulin at 1 μmol/L were found to be suitable conditions for IR modeling. SCFAs increased glucose consumption and expression level of protein AMPK,AKT,PI3K,GLUT 2 and GLUT 4,as well as mRNA. In conclusion,SCFAs derived from the digestion of potato RS could improve IR in cells by enhancing glucose transport through the AMPK,PI3K/AKT signalling pathways and GLUT 2/GLUT 4 target proteins,increasing cellular glucose consumption,while exhibiting low effective concentrations and high biosafety.
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