Objective: The effect of the critical medium components and culture conditions on recombinant endochitinase production from pichia pastoris was investigated in this study. Methods: With the endochitinase activity as the target, the concentrations of several important factors, methanol, oleic acid, Tween-80, PTM1, cell concentration, pH and medium volume were optimized, respectively. Results: The optimal concentrations of methanol,oleic acid,Tween-80 and PTM1 were 0.5％, 0.05％, 0.4％ and 0.6％, respectively. The optimal pH, cell proportion and medium volume were 6.0,3∶1 and 25 mL/100 mL, respectively. The highest activity of endochitinase was achieved at 72 h in the optimal medium with activity of 89.3 U/mL, which was 3.26-fold higher than that in the control medium. Conclusion: The results provided theoretical and technological support for the waste chitin-degrading into highly physiological active chitooligosaccharide.
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万方数据
维普
