Objective:To extract the polysaccharides from polygonatum and to investigate the effects of the polygonatum polysaccharide (PP) on immunological function in immunosuppressed mice, and provide the experimental basis for develop and application of PP. Methods:The PP was extracted from dried roots of polygonatum by hot water extraction, ethanol precipitation and deproteinization with Sevage method. The mice were established as immunosuppressed model by peritoneal injecting cyclophosphamide. The mice were administered once daily by intragastric administration with PP solution dose of 200 and 600 mg/kg respectively for 7 days. The effects of PP on model mice were measured following these indexes, the weights of immune organs, the serum hemolysin, and phagocytic activity of peritoneal macrophages. Results:The extraction rate of PP was(9.20±0.15)% and content(84.65± 1.31)% in the condition of the ratio of solid to liquid 1:15, extraction temperature 90℃ and time 2 h for two times. While compared with immunosuppressive model mice groups, the PP groups can increase the thymus and spleen indexs, phagocyte-percentage and phagocytic-index of peritoneal macrophages significantly(P<0.05), and can promote the formation of hemolysin significantly(P<0.05). Conclusions:The PP can obviously improve immunological function of the immunosuppressive model mice induced with cyclophosphamide. The polygonatum contain high content of polysaccharide that is worthy of development.
polygonatum polysaccharide(PP)extractingimmunosuppressive model miceimmunological activity
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NSTL
维普
万方数据
