首页|响应面优化银杏叶中黄酮的提取工艺

响应面优化银杏叶中黄酮的提取工艺

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为研究银杏叶中黄酮的提取工艺条件,以黄酮提取量为指标,采用乙醇回流法做实验.通过单因素试验及响应面分析,探讨乙醇体积分数、固液比、提取时间和提取温度对黄酮提取量的影响.同时应用响应面交互作用,分析优化得出该方法的最佳提取条件是:乙醇体积分数83%,固液比1∶22 g/mL,提取时间98 min,提取温度71℃.在此条件下银杏叶黄酮提取量为18.38 mg/g.将样品纯化,用红外光谱对其结构进行表征.
Optimization of Flavonoids from Ginkgo Biloba Using Respone Surface Analysis
This paper studies the technological conditions of extracting flavonoids from Ginkgo leaves. Extraction of flavonoids as an indicators, a single factor experiment and responsesurface method were used in ethanol extraction method. The extraction of flavonoids as an indicator, the use of ethanol reflux experiment, the single factor experiments and response surface analysis of the ethanol concentration, solid-liquid ratio, extraction time and ftxtraction temperature on the extraction of flavonoids. Analysing by Response Surface Method obtaines the optimum technological condition for Ginkgo leaves; ethanol concentration is 83%, the mass ratio is 1:22 g/mL, extracting time is 98 min and extracting temperature is 71 ℃. Under this condition the extraction of flavonoids is 18.38 mg/g. At the same time, the purified sample are characterized by IR spectra.

flavonoidsextractionanalysis of response surface methodcharacterization

林建原、季丽红

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浙江万里学院生物与环境学院 浙江宁波315100

黄酮 提取工艺 响应面分析 结构表征

浙江省自然科学基金浙江省科技厅新苗人才计划

Y21108652012R419020

2013

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCDCSCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2013.13(2)
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