Fermentation Condition Optimization of Aspergillus niger Producing Extracellular Glucose Oxidase
A strain producing extracellular glucose oxidase named Aspergillus Niger 1504 was screened from soil samples and its mutant UN Ⅱ 021 was obtained by mutation breeding.Higher activity of extracellular glucose oxidase was obtained optimizing the medium composition and culture conditions with the best conditions as followed:10％ glucose as carbon source,0.5％ beef extract peptone and 0.5％ sodium nitrate as nitrogen source,the amount of calcium carbonate 30g/L,inoculation amount 0.05 (V/V).The optimum temperature and pH were 29 ℃ and 5.2 respectively,with shaking speed was 240 r/min.The production of extracellular glucose oxidase by UN Ⅱ 021 could be 363.04 U/mL by the end of the Forth day,which was 2.14 times more than the original one.
NETL
NSTL
万方数据
维普
