首页|不同枸杞中多糖含量与结构特征的对比研究

不同枸杞中多糖含量与结构特征的对比研究

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目的:比较对不同产地红果与黑果枸杞中枸杞多糖的含量与结构特征.方法:采用中药指纹图谱技术,相似度分析法和聚类分析法对3个产地的红果及黑果枸杞中多糖样品的红外(IR)、高效凝胶渗透色谱(HPGPC)、单糖组成的柱前衍生化-高效液相(PCD-HPLC)指纹图谱进行比较分析.结果:1)黑果枸杞多糖的中性糖含量与红果枸杞相比无显著差异,酸性糖含量极显著地高于红果枸杞.2)不同产地、品种红果与黑果枸杞中的枸杞多糖结构相似;3)产地因素对枸杞多糖的糖环形式和糖苷键类型、单糖组成及其物质的量比的影响大于品种因素.结论:黑果枸杞中酸性多糖的含量极显著地高于红果枸杞中的酸性多糖,而两种枸杞中中性多糖含量无显著差异,两种枸杞中多糖的结构基本相似.采用中药指纹图谱技术结合相似度分析和聚类分析法,可快速鉴别枸杞多糖并应用于枸杞及相关产品的多糖的质量控制.
Comparative Study of the Content and Structural Features of Polysaccharides from Different Lycium
Objective:Analyzing the similarities and differences between polysaccharides of L.barbarum and L.ruthenicum fruits from different locations.Methods:IR and HPLC spectra of six Goji polysaccharide samples were compared and analyzed by fingerprint,similarity and cluster analysis.Results:1) The total neural carbohydrate contents from different samples have no significant difference,while acidic carbohydrate contents of L.ruthenicum Goji polysaccharides were significantly higher than that in the polysaccharides from L.barbarum fruits.2) The structural features of polysaccharides extracted from L.barbarum or L.ruthenicum fruits were similar.3) Location was the most important factor affecting the sugar ring forms,glycosidic bond types,monosaccharide composition and its molar ratio of Goji polysaccharide.Conclusion:The results of this study revealed that acidic polysaccharides contents of L.ruthenicum Goji were very significantly higher than that from L.barbarum fruits,while neutral polysaccharides contents of them have no significant difference,and the structural features of polysaccharides extracted from L.barbarum or L.ruthenicum fruits were similar.TCM fingerprint technology combined with similarity analysis and cluster analysis can quickly identify Goji polysaccharide and control the quality of polysaccharides from Goji as well as related products.

Lycium barbarumLycium ruthenicumpolysaccharidehierarchical clustering analysis

徐金楠、刘玮、刘春晶、柴银、高向东、姚文兵

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中国药科大学生命科学与技术学院 南京 210009

红果枸杞 黑果枸杞 多糖 聚类分析

国家科技重大专项国家自然科学基金国家级大学生创新创业训练计划项目

2012ZX09502001-00431301473

2015

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCDCSCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2015.15(4)
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