Optimization of Ultrasound-assisted Extraction of Melanin by Auricularia auricula Response Surface Method
melanin is a biologically active substance in Auricularia auricula with anti-aging and regulating immune function.The thesis tells new process conditions of ultrasonic assisted extraction of melanin,providing the technical basis for further development and utilization of Auricularia auricula resources.By Auricularia auricula powder materials,and by single-factor test filtering out sodium hydroxide concentration,ultrasonic power,ultrasonic time and solid-liquid ratio of 4 factors,then after 3 Box-Behnken single factor design using response surface methodology to optimize conditions of ultrasonic assisted extraction of melanin.Experimental results show that the optimum extraction technological conditions of melanin is the sodium hydroxide concentration 0.5 mol· L-1,the ultrasonic power 348W,ultrasonic time 9min and solidliquid ratio 1∶21 g/mL.Melanin absorbance of Auricularia auricula in this condition was 0.787,Extraction rate of the ultrasonic assisted extraction of melanin from A uricularia auricula increased by 26.94％ compared with solvent extraction.The method of ultrasonic-assisted extraction of melanin is accurate,solvent-saving and efficient.
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