黑李提取物对结肠癌Caco-2细胞增殖和调亡的影响
Effect of Black Plum Extract on Anti-proliferation and Apoptosis
彭吕杨 1胡冬梅 1薛照辉1
作者信息
摘要
采用80％丙酮浸提法制备黑李粗提物.通过MTT法测定黑李提取物对Caco-2细胞增殖的抑制效果,Annexin V-FITC/PI双染色法、PI染色法、酶联免疫分析法分别检测细胞凋亡、细胞周期以及凋亡蛋白相对表达量变化,探究黑李提取物对Caco-2细胞凋亡信号转导通路的影响.与空白对照组相比,不同浓度的黑李提取物(0.2～2.0 mg/mL)对Caco-2细胞增殖均有明显的抑制作用,随浓度的增大,Caco-2细胞凋亡率明显升高,呈现显著的剂量-效应关系.提取物处理细胞72h后,使细胞周期G1期和G2延长,CytC、caspase-9、caspase-3等凋亡蛋白的相对表达量明显上升.提示黑李提取物通过阻滞细胞周期,诱导线粒体通路介导的细胞凋亡.本结果为将黑李开发为抗癌功能性食品提供了科学依据.
Abstract
To explore the anti-cancer activity and apoptosis of black plum against human colon cell Caco-2.Method: black plum extract was acquired by 80％ acetone extraction, and inhibition effect on cell proliferation was determined by MTT method.In addition, to determinate cell apoptosis, cell cycle and expression of apoptosis protein, Annexin V-FITC/PI double staining assay, PI staining assay and ELISA were applied respectively.Result: Compared with control group, black plum extract had a significant inhibition on Caco-2 cell proliferation at different concentrations(0.2～2.0 mg/mL), and a dose-effect relationship can be observed.After treated with black plum extract for 72 h, cell cycle G1 and G2 periods were prolonged.The relative expressions of CytC, caspase-9, caspase-3 related to apoptosis showed obvious increases which proved that black plum extract can induce apoptosis via mitochondrial pathway.Conclusion: the result provides a scientific and theoretical basis to the development of anti-cancer functional food.
关键词
黑李/人结肠癌细胞Caco-2/细胞凋亡/细胞周期/线粒体通路Key words
black plum/human colon cell Caco-2/apoptosis/cell cycle/mitochondrial apoptotic pathway引用本文复制引用
出版年
2016
NETL
NSTL
维普
万方数据