The alterations of several enzymes activities in ICR mice were studied by intraperitoneal injection of muscle protein extracts from Chinese shrimp (Fenneropenaeus chinensis).The activities of SOD,POD,CAT,GSH-Px,GST,NOS (iNOS) and the concentration of PLA2 as well as the relative mRNA expression level of Ca2+-ATPase in the liver,kidney,spleen and sera were analyzed.The results showed that the sIgE levels in the serum of mice were significantly increased after the shrimp protein extracts injection,indicating that the allergic model of mice was successfully established.Besides,the activities of SOD,POD,CAT,GSH-Px,GST and NOS (iNOS) in the liver,kidney,spleen and sera were remarkably enhanced in sensitized mice,so as the concentration changes of PLA2.However,the mRNA expression of Ca2+-ATPase was decreased after sensitization.Our results may provide some evidence for evaluation of allergic reactions in mouse model and better understanding the mechanism of allergy of Chinese shrimp extracts.
NETL
NSTL
万方数据
维普
