Antioxidant and Antineoplastic Activities of Carboxymethyled Polysaccharide from Fruits of Phyllanthus emblic L.
A water-soluble polysaccharide extracted from phyllanthus emblica (EPS) was carboxymethylated in the system of sodium hydroxide-isopropanol-sodium monochloroacetate and the activity of both of the polysaccharide about scavenging free radical,NO2-and inhibiting proliferation effect of HepG-2 as well as cell apoptosis were comparative investigated for the sake of evaluating CM-EPS activity.Characteristics of the spectrum (FT-IR) showed the modification was successful,carboxymethylation of the polysaccharide from emblica (CM-EPS) was prepared.CM-EPS exhibited a declined scavenging effect on ABTS radical but exhibited a markedly enhanced scavenging effect on DPPH in high concentrations compared with the native polysaccharide.In addition,it also revealed an outstanding ability on scavenging NO2-and enhanced inhibitory effect against the proliferation of Human hepatoma cell HepG-2 remarkable.Furthermore,agarose gel electrophoresis demonstated HepG-2 cells were apoptosised with the disposal of CM-EPS which indicated that CM-EPS might be used as a novel functional food for human consumption.
polysaccharide of emblicacarboxymethylationDPPHABTSNO2-HepG-2
NETL
NSTL
万方数据
维普
