Screening of Lactic Acid Bacteria in Chinese Traditional Pickled Vegetables with High Production of AI-2 Signal Molecule and Its Probiotic Properties
LuxS/AI-2,as an interspecific communication quorum sensing(QS)system,is commonly found in lactic acid bacteria(LAB),which can regulate the probiotic properties of LAB,including biofilm formation,acid tolerance,bile salt tolerance,cells adhesion.In this study,LAB with production of AI-2 signal molecule was screened using strain Vibrio harveyi BB170 reporting method.The expression of luxS gene was detected by PCR sequencing.Moreover,the probiotic properties of high-yield AI-2 LAB were evaluated by acid resistance,bile salt resistance,antibacterial and cells adhe-sion.The results showed that ten strains of LAB producing AI-2 were screened from two hundred strains LAB.Among them,Lactobacillus plantarum SCT-2 had strong ability to produce AI-2 signal molecule.The luxS gene was further ver-ified exist in strain SCT-2.The survival rates of acid and bile salt resistant in strain SCT-2 were 14％-92％and 63％-89％,respectively.The antibacterial diameter of strain SCT-2 ranged from 17.50 mm to 19.43 mm.The number of adhe-sion cells of strain SCT-2 was 102 CFU/cell.Compared to LAB with low production AI-2,the biofilm formation,acid tolerance,bile salt tolerance,inhibitory rate and cell adhesion of strain SCT-2 increased by 111.4％,8％,15％,26.7％and 197％,respectively.These results indicated that luxS gene was the key gene of AI-2 signal synthesis.LuxS/AI-2 quorum sensing system improves the biofilm forming ability,acid tolerance,bile salt tolerance antibacterial ability and cells adhesion of LAB.It will provide a theoretical basis for the development of LAB biological agent with good probiotic properties.
Lactobacillus plantarumquorum sensingAI-2 signal moleculeprobiotic properties
万方数据
维普
