Effect of Rice Bran Phenolic Extracts on Abnormal Glucose Metabolism and Oxidative Stress Induced by High-fat Diet in Mice
Objective:This study was conducted to investigate the effects of rice bran phenolic extracts(RBPE)supple-mentation on glucose metabolism and oxidative stress in high-fat diet induced mice,which could provide theoretical basis for revealing the effect of rice bran on glucose metabolism.Methods:After 1 week of acclimatization,mice were ran-domly divided into 4 groups:normal control group,high-fat diet group,low-dose RBPE group(200 mg/kg,HF+LP group)and high-dose RBPE group(400 mg/kg,HF+HP group).The experiment lasted for 16 weeks.The organ-body ratio,epididymal fat morphology,fasting serum glucose level,oral glucose tolerance and the levels of oxidative stress were measured.Results:RBPE dose-dependently decreased the organ-body ratio,fasting blood glucose and ameliorated the impaired glucose tolerance induced by high-fat diet.Compared with the high-fat diet group,organ-body ratio of the liver of HF+LP and HF+HP groups were decreased by 15.75％and 39.61％,respectively,the organ-body ratio of epi-didymal adipose tissue decreased by 23.03％and 39.83％,respectively,the organ-body ratio of renal peritoneal adipose tissue decreased by 43.68％and 56.83％,respectively,fasting blood glucose levels decreased by 14.06％and 20.21％,and the area under the glucose tolerance curve decreased by 19.06％and 23.81％,respectively.Further studies showed that low and high doses of RBPE can significantly reduce serum and liver tissue MDA content and increase SOD activity in high-fat diet induced mice.Compared with the high-fat diet group mice,serum and liver MDA content in HF+HP group mice were decreased by 48.93％and 38.68％,respectively,SOD activity increased by 80.44％and 67.05％,re-spectively;RBPE treatment could also increase antioxidant enzyme activities such as catalase(CAT)and glutathione per-oxidase(GSH-px)and the content of antioxidant glutathione(GSH)in liver tissue of high-fat diet induced mice(P＜0.05).Compared with the high-fat diet group mice,CAT,GSH-px activity and GSH content in HF+HP group mice were increased by 48.49％,31.67％and 123.90％,respectively.Conclusion:RBPE could alleviate the impaired glucose metabolism induced by high-fat diet,which might be related to its inhibition effect on oxidative stress.
万方数据
维普
