In this study,It combined saltatory rolling circle amplification(SRCA)with fluorescence technology to estab-lish a real-time fluorescence SRCA technique for detection of honey adulterated rice syrup.Three DNA extraction proto-cols were evaluated,four specific genes of rice(PDL,SPS,rbcL,GOS9)were used as target sequences to design primers,suitable primers were screened,amplification reaction conditions were optimized,and a method for detection of honey adulterated rice syrup was established in combination with fluorescence technology.The results showed that the sensitivity of the established real-time fluorescence SRCA method for the detection of rice DNA was 8.45×101 fg/μL,and its good specificity was confirmed by the specificity evaluation.The linear relationship between the logarithm of the pro-portion of adulteration and the Ct value was established in the manual simulated adulteration detection test,and the lin-ear equation was y=6.618x+7.651(R2=0.993),which can accurately detect the components of rice syrup in honey down to 1％.The method is sensitive,with low detection limit,and can quickly and accurately quantify the adulteration of honey with rice syrup,which provides a new idea for the rapid quantitative detection of honey adulteration.
关键词
蜂蜜掺伪/深加工食品/跨越式滚环等温扩增(SRCA)
Key words
adulterated honey/deeply processed food/saltatory rolling circle amplification(SRCA)
维普
万方数据