The Role of Jasmonate in L-Glutamate Induced Tomato Fruit Resistance
To decipher the role of jasmonate in chemical elicitor L-glutamate induced resistance to pathogens,tomatoes were treated with 0.1 mg/mL L-glutamate and/or 0.1 mmol/L methyl jasmonate.After inoculating with Alternaria alternata spore suspension,disease incidence of fruit was observed.The effect of L-glutamate on jasmonate biosynthesis and sig-naling pathways were analyzed by reverse transcription quantitative real-time polymerase chain reaction at the transcrip-tional level.The results showed that the disease incidence decreased by 35％in L-glutamate treatment compared with the control,while there was no significant difference between L-glutamate and the control in methyl jasmonate group.Con-currently,allene oxide synthase,allene oxide cyclase,coronatine insensitive 1,transcription factor MYC2 and protease inhibitor PⅠ-Ⅱ were down-regulated by L-glutamate,accompanied by higher expression level of the negative regulator JAZ1.Collectively,the inhibition of JA pathway might be involved in L-glutamate induced resistance to Alternaria alter-nata in tomato fruit.These findings provided a better understanding for L-glutamate mediated resistance,leading to more sufficient theoretical basis for its commercial application.
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