氮掺杂碳量子点荧光法检测猪肉中的四环素
Fluorescent Determination of Tetracycline in Pork Based on Nitrogen-doped Carbon Quantum Dots
周彤 1毋晨 1赵文 1李明 2彭汝艳 1周茜1
作者信息
- 1. 河北农业大学食品科技学院 河北保定 071001
- 2. 河北农业大学理学院 河北保定 071001
- 折叠
摘要
四环素(TC)作为一种广谱抗生素,用于预防和治疗细菌性疾病,以及作为饲料添加剂促进动物生长.本文旨在建立一种菠萝皮来源的氮掺杂碳量子点(N-CQDs)荧光猝灭法快速检测TC.以菠萝皮和二乙烯三胺为原料,通过水热法制备高量子产率的蓝色荧光N-CQDs.通过透射电子显微镜、傅里叶红外光谱、X射线光电子能谱对N-CQDs进行结构表征.结果表明,N-CQDs的平均粒径为3.53 nm,主要由C、N、O 3种元素组成,表面具有含氧和含氮基团.主要猝灭机理为静态猝灭和内滤效应.在最优试验条件下,该方法在0.3～40 μg/mL范围线性良好,检出限为89.26 μg/L.该检测方法被用于猪肉中四环素的检测,回收率在98.40％～106.02％之间,相对标准偏差1.29％～3.88％.
Abstract
As a common antibiotic,tetracycline(TC)is extensively used in the prevention and treatment bacterial dis-eases in animals and is also used as a feed additive to promote the growth of animals.In this paper,a fluorescence quenching method based on nitrogen doped carbon quantum dots(N-CQDs)derived from pineapple peel was established for the rapid detection of TC.The blue fluorescent N-CQDs with high quantum yield were prepared by the hydrothermal method using pineapple peel and diethylenetriamine as precursors.The structure of N-CQDs was characterized by trans-mission electron microscope,fourier infrared spectroscopy and X-ray photoelectron spectroscopy.The results showed that the average particle size of N-CQDs was about 3.53 nm,which was mainly composed of C,N and O,and the surface has oxygen-containing and nitrogen-containing groups.The fluorescence of N-CQDs was effectively quenched by static quenching and internal filtering effect.Under the optimal experimental conditions,the method had good linearity within 0.3-40 μg/mL,and the detection limit was calculated to be 89.26 μg/L.Moreover,the detection method has been applied to the determination of TC in pork,the recovery rate was 98.40％-106.02％.The results showed that the detection method could be applied to the rapid determination of TC in pork.
关键词
菠萝皮/氮掺杂碳量子点/荧光检测/四环素Key words
pineapple peel/nitrogen doped carbon quantum dots/fluorescent detection/tetracycline引用本文复制引用
基金项目
河北省现代农业产业技术体系生猪创新团队项目(HBCT2018110205)
出版年
2024
NETL
NSTL
万方数据