Fluorescent Determination of Tetracycline in Pork Based on Nitrogen-doped Carbon Quantum Dots
As a common antibiotic,tetracycline(TC)is extensively used in the prevention and treatment bacterial dis-eases in animals and is also used as a feed additive to promote the growth of animals.In this paper,a fluorescence quenching method based on nitrogen doped carbon quantum dots(N-CQDs)derived from pineapple peel was established for the rapid detection of TC.The blue fluorescent N-CQDs with high quantum yield were prepared by the hydrothermal method using pineapple peel and diethylenetriamine as precursors.The structure of N-CQDs was characterized by trans-mission electron microscope,fourier infrared spectroscopy and X-ray photoelectron spectroscopy.The results showed that the average particle size of N-CQDs was about 3.53 nm,which was mainly composed of C,N and O,and the surface has oxygen-containing and nitrogen-containing groups.The fluorescence of N-CQDs was effectively quenched by static quenching and internal filtering effect.Under the optimal experimental conditions,the method had good linearity within 0.3-40 μg/mL,and the detection limit was calculated to be 89.26 μg/L.Moreover,the detection method has been applied to the determination of TC in pork,the recovery rate was 98.40％-106.02％.The results showed that the detection method could be applied to the rapid determination of TC in pork.
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