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顶温对红心曲微生物群落结构及理化性质的影响

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制曲温度能显著影响大曲的理化指标及微生物群落结构,因而大曲分为高温曲、中温曲和低温曲.在清香大曲制作中小范围改变温度,对其微生物及理化性质的影响并不清楚.本研究比较不同顶温对红心曲微生物群落结构及其理化性质的影响.结果表明:两组红心曲中微生物多样性没有显著区别,而属水平微生物群落结构发生明显变化.顶温升高5℃,红心曲真菌中嗜热子囊菌由原来的64.7%增至78.4%,扣囊腹膜酵母则由33.3%降至15.2%,显著增加的还有产淀粉酶的曲霉和根霉.顶温升高后显著增加的细菌有醋酸菌、魏斯氏菌和葡萄球菌等,均有利于白酒风味的形成.大曲中一般少量的放线菌类糖多孢菌由26.7%降至8.8%.理化性质方面,顶温升高5℃大曲糖化力由596.9 U降至516.1 U,液化力由1.26U降至1.05 U,但下降均不显著;发酵力和酒化力有所增加,发酵力由0.74 U增至2.27 U,酒化力由7.1%vol提高到8%vol.结合实际情况提高顶温,能优化大曲微生物群落结构,改善综合质量,研究结果为红心曲的优质高产提供理论基础.
Effect of Peak-temperature on the Microbial Community Structure and Physicochemical Properties of Hongxinqu
Temperature is used for classifying Daqu as high temperature Daqu,medium temperature Daqu and low tem-perature Daqu,since it significantly affects the physical and chemical indexes and microbial community structure of Daqu.The effect of temperature varied in a small range during the manufacture of Daqu for Light-flavor baijiu brewing on its microbial and physicochemical properties is still unclear.In this study,the effect of microbial community structure and physicochemical properties of Hongxinqu by different peak-temperature were compared.The results showed that there was no significant difference in microbial alpha diversity between the two groups of Hongxinqu from different peak tem-perature.However,the microbial community structure at the genus level has changed significantly.In Hongxinqu samples from peak-temperature rised 5℃,Thermoascus increased from 64.7%to 78.4%,and Saccharomycopsis fibuligera de-creased from 33.3%to 15.2%,Eurotium and Rhizopus producing amylases were also significantly increased.Similarily,in Hongxinqu samples from peak-temperature rised 5 ℃,several bacteria genus significantly increased included Acetobacter,Weissella,and Staphylococcus,all of which were beneficial to the formation of Baijiu flavor,while Actinomycete Sac-charopolyspora which is little in general Daqu samples decreased from 26.7%to 8.8%.About of the physicochemical properties,when the peak-temperature increased by 5℃,the saccharification of Daqu decreased from 596.9 U to 516.1 U and the liquefaction decreased from 1.26 U to 1.05 U,but the decrease was not significant;The fermenting power and liquor-producing power have increased,with the fermenting power increasing from 0.74 U to 2.27 U and the liquor-pro-ducing power increasing from 7.1%vol to 8%vol.About of the physicochemical properties,the temperature increment of a small range did not significantly reduce the saccharification power and liquefaction power,while the fermenting power and liquor-producing power were increased.The overall results showed that increase of peak-temperature during Hongxin-qu making process according to the actual situation can effectively optimize the microbial community structure of Hongx-inqu and improve its comprehensive quality,which provided a theoretical basis for Hongxinqu production of high quality and highyield.

Hongxinqupeak-temperaturemicrobial communityphysicochemical properties

王昊乾、刘昌录、顾铁刚、任晓壮、唐艺婧、张秀红

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山西师范大学生命科学学院 太原 030002

内蒙古阿尔一一九八酒业有限公司 内蒙古乌兰浩特 137400

山西师范大学食品科学学院 太原 030002

红心曲 顶温 微生物群落 理化性质

山西省自然科学基金山西省基础研究计划山西省基础研究计划

201901D111286202103021223255202203021221135

2024

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2024.24(3)
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