In order to explore the changing of lignin in bamboo shoots,the Phyllostachys praecox were stored at room temperature(25℃)and low temperature(4℃),and the enzyme activity and relative gene expression related to changes in lignin content and components were studied.The results showed that:at room temperature,the bamboo shoots showed changes such as yellowing and decay after stored for 6 days;under low temperature storage conditions,the bamboo shoots showed yellowing and decay until the 24th day.Stored at low temperature for 6 days,the lignin content was 13.22 mg/g,and increased to 26.31 mg/g on the 24th day.The total amount of amino acids did not change much under low-temperature storage conditions,and it showed a slow downward trend at room temperature.On the 6th day,the amino acids decreased by 21.78％at the room-temperature storage.Enzyme activities such as phenylalanine ammonia lyase(PAL),peroxidase(POD),cinnamyl alcohol dehydrogenase(CAD),and cinnamic acid-4-hydroxylase(C4H)showed a significant upward trend with extending storage time.Enzyme activities under room temperature storage were higher than those under low temperature storage.Low temperature reduced the activity of bamboo fiber-related enzymes by inhibiting the expression of genes related to bamboo lignin formation,thereby slowing down the process of lignification.Under the low temperature,the number of genes related to down-regulated expression increased with the extending of storage time.The most significant down-regulation of genes were observed at 24 d,while the amount of up-regulated genes was rela-tively small,with the smallest number observed at 12 d.The relative expression levels of LAC-10,POD-10,HCT-10,CAD-10,and CCR-10 genes showed an overall upward trend under low temperature storage,while the relative expres-sion level of COMT-10 gene decreased continuously.This study provides a theoretical basis for controlling post-harvest quality of bamboo shoots.
维普
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