Quorum sensing(QS)is a communication pattern between bacteria,hence the inhibition of bacterial QS sys-tem could be used as a new strategy to the control the spoilage caused by bacteria,and to some extent could avoid the bacteria drug resistance.In the study,dandelion water extract mediated biosynthesis Ag nanoparticles(D-AgNPs)was prepared with AgNO3 as silver source and dandelion water extract as reducing agent.Its structure was characterized by ultraviolet visible absorption spectroscopy(UV-vis),dynamic light scattering(DLS),Zeta potential,X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM)and X-ray energy spectrum(EDS).It was found that the synthesized D-AgNPs were spherical,with an average particle size of 13.9 nm and Zeta potential of(-14.07±0.25)mV.The effects of D-AgNPs on the growth of two Hafnia strains and quorum sensing regulat-ed virulence factors such as biofilm,AHLs,proteases,exopolysaccharides,swimming and swarming motilities were deter-mined.It was found that the minimum inhibitory concentration of D-AgNPs against Hafnia was 128 μg/mL,and could inhibit the formation of the biofilms of Hafnia at 1/16～1/2 MIC(8-64 µg/mL),whereas promoted its biofilm formation at 1/128～1/32 MIC(1-4 μg/mL).D-AgNPs showed different inhibition abilities on the AHLs and protease production,as well as swimming and swarming motilities;however,sub-MIC D-AgNPs promoted the production of exopolysaccharides.It is proved that D-AgNPs could be used as quorum sensing inhibitor.
关键词
银纳米颗粒/哈夫尼亚菌/群体感应/绿色合成/三文鱼
Key words
Ag nanoparticles/Hafnia/quorum sensing/green synthesis/salmon
维普
万方数据