This study took Limosilactobacillus reuteri F22A089 as an example to explore domestication strategies to en-hance its nucleoside degradation ability,which provided theoretical basis for obtaining strains with high uric acid lower-ing ability and targeted domestication of specific functions of probiotics.Two methods of in vitro and in vivo domestica-tion were used to domesticate the ability of probiotics to degrade nucleosides.The results showed that the in vitro do-mestication method could not rapidly improve the nucleoside degradation ability of probiotics strains,and the nucleoside degradation ability of probiotics strains remained unchanged after 3 weeks of domestication.The study on in vivo domes-tication of probiotic strains by high-uric acid intestinal environment in mice driven by diet found that the domestication of Limosilactobacillus reuteri F22A089 increased the degradation ability of nucleoside,and the degradation rate of guano-sine in some strains reached twice that of the original strain,indicating that it is feasible to target domestication of pro-biotic strains by host intestinal selection pressure.Whole genome and transcriptome sequencing and mutation site analysis were performed in domesticated strains.Three mutant genes(pbuG_1,rihB and yusV)related to uric acid lowering a-bility were noted.The mutations of these functional genes were closely related to the enhancement of uric acid lowering ability in domesticated strains.This study was the first to domesticate strains in vivo and improve their ability to degrade uric acid,providing a feasible domestication method for domesticating probiotics through the host gut and enhancing their probiotic efficacy.
关键词
益生菌/定向驯化/体内驯化/降尿酸/罗伊氏粘液乳杆菌F22A089
Key words
probiotics/targeted domestication/in vivo domestication/uric acid degradation/Limosilactobacillus reuteri F22A089
维普
万方数据