Objective:To investigate the protective effect of Ganoderma atrum polysaccharide(PSG-1)against lipopolysaccharide(LPS)-induced damage in intestinal epithelial cell(IEC-6)and its underlying mechanisms.Methods:The intervention effects of PSG-1 on IEC-6 cells were investigated using a LPS-induced intestinal epithelial cell IEC-6 injury model,and the effect of PSG-1 intervention on cell viability was assessed using the cell counting kit-8(CCK-8)assay.Western blot technology was employed to investigate the alterations in the expression of intestinal tight junction proteins and cyclooxygenase-2(cox-2)in the cells.Transcriptome sequencing analysis was conducted to explore the po-tential protective mechanisms of PSG-1,followed by experimental validation.Results:Intervention with PSG-1 signifi-cantly enhanced cell viability and the expression of intestinal barrier proteins,including ZO-1,Claudin-1,and Occludin.Moreover,PSG-1 exhibited an inhibitory effect on the abnormal upregulation ofcox-2 induced by LPS.Transcriptome se-quencing analysis,scratch assay,and western blot experiments validation demonstrated that PSG-1 significantly enhanced cell migration ability while suppressing the expression of pro-apoptotic proteins,including Bax,Caspase-3,and Caspase-9.Conclusion:PSG-1 exhibited significant improvement in LPS-induced damage in IEC-6 cell.Cell migration and apop-tosis might be key mechanisms through which PSG-1 exerted its protective effects.
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