首页|复合抗冻剂的研制及对鮰鱼肌原纤维蛋白的抗冻效果

复合抗冻剂的研制及对鮰鱼肌原纤维蛋白的抗冻效果

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以鲫鱼鱼鳞为原料,用胰蛋白酶酶解法获得抗冻粗肽.以抗冻粗肽、海藻糖和褐藻寡糖为因素,运用{3,3}单纯形格子法进行配方设计,以冷冻24 h后嗜热链球菌的存活率为抗冻活性筛选指标,获得复合抗冻剂理论模型配方.选用复合抗冻剂最优组(K1、K2)和理论模型配方组(K3)进行冷冻鮰鱼肌原纤维蛋白试验,验证复合抗冻剂的抗冻效果.结果表明:抗冻粗肽组嗜热链球菌的存活率达57.11％.与大豆卵磷脂互作的红外光谱显示:抗冻粗肽可能与磷脂亲水头部和水之间发生相互作用,有利于稳定膜结构.K1和K2嗜热链球菌的存活率在80％以上,K3嗜热链球菌的存活率高达92％.添加K1、K2和K3复合抗冻剂后蛋白质表面疏水性显著降低(P＜0.05),蛋白质溶解度显著升高(P＜0.05).K1和K2的α-螺旋和β-折叠含量显著下降(P＜0.05),K2和K3的r值显著降低(P＜0.05).结论:理论模型K3是具有高抗冻活性,可抑制蛋白质聚集和维持蛋白质结构稳定的一种良好的水产品复合抗冻剂.本研究为复合抗冻剂的开发提供新思路,为后续开发鮰鱼预制菜冷冻产品提供了理论参考.
Preparation of Composite Antifreeze and Its Antifreeze Effect of Channel Catfish Myofibrillar Protein
In this study,antifreeze crude peptide was obtained from crucian carp fish scales by trypsin enzyme digestion.And the formulation was designed by using {3,3} simplex lattice method with antifreeze crude peptide,trehalose and al-ginate oligosaccharides as factors.The survival rate of Streptococcus pyogenes(S.pyogenes)after 24 h of freezing was used as the screening index for antifreeze activity to obtain the theoretical model formulation of the compound antifreeze.The optimum group of composite antifreeze(K1,K2)and the theoretical model formulation group(K3)were further se-lected for the frozen channel catfish(Ictalurus punctatus)myofibrillar protein test to verify the antifreeze effect of the composite antifreeze.The results showed that the survival rate of S.pyogenes in the antifreeze crude peptide group reached only 57.11％.The infrared spectra of the interaction with soy lecithin showed that the crude antifreeze peptide might interact with the hydrophilic head of phospholipids and water,which could help to stabilize the membrane struc-ture.The survival rate of S.pyogenes reached more than 80％in the K1 and K2 groups,and as high as 92％in the K3 group.The hydrophobicity of the protein surface was significantly reduced(P＜0.05)and the protein solubility was significantly increased(P＜0.05)by the addition of K1,K2 and K3 complex antifreeze.The α-helix and β-fold content of K1 and K2 were significantly decreased(P＜0.05);and the r-values of K2 and K3 were significantly decreased(P＜0.05).In summary,the theoretical model K3 is a good aquatic products composite antifreeze with high antifreeze activi-ty,which can inhibit protein aggregation and maintain the stability of protein structure.This study provides a new idea for the efficient development of composite antifreeze,and provides a theoretical basis for the subsequent development of catfish pre-prepared vegetables frozen products.

crude antifreeze peptidethe simplex lattice methodantifreezecomposite antifreezemyofibrillar protein

逯晓燕、丛海花、沈益诚、赵勇、陈继伟、江雨桐

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大连海洋大学食品科学与工程学院 辽宁大连 116023

中科荣耀(苏州)生物科技有限公司 江苏苏州 215152

苏州农业职业技术学院食品科技学院 江苏苏州 215008

抗冻粗肽 单纯形格子法 抗冻性 复合抗冻剂 肌原纤维蛋白

国家自然科学基金青年科学基金辽宁省教育厅高等学校基本科研面上项目江苏省高职院校教师专业带头人高端研修项目江苏省高等学校"青蓝工程"中青年学术带头人项目苏州市乡村振兴软科学研究计划重点课题青年教师科研能力提升计划(2022)江苏省职业院校学生创新创业培育计划(2023)

31701630LJKZ07152023GRFX061SN-XCZX-2023-04BS[2022]18

2024

10.16429/j.1009-7848.2024.04.019

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCD北大核心EI
影响因子：1.079
ISSN：1009-7848
年,卷(期)：2024.24(4)
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