Studies on Preparation and Antioxidant Activity of Artemisia integrifolia Polysaccharides
Objective:Isolate and purify polysaccharides from Artemisia integrifolia and to investigate their antioxidant ac-tivity.Methods:The optimum extraction process was obtained by ultrasonic-assisted enzymatic extraction of polysaccha-rides from Artemisia integrifolia using response surface methodology.Results:The optimum extraction process was ob-tained as follows:ultrasonic power 300 W,material-liquid ratio 1∶30 g/mL,ultrasonic time 46 min,addition amount of cellulase 2.0％,addition amount of pectinase 3.0％,and under this condition,polysaccharide yield 7.67％.A total of six polysaccharide fractions were obtained by separation and purification using DEAE-52 chromatography column.Antioxidant activity studies on both AIP-2 and AIP-3 fractions showed that both AIP-2 and AIP-3 treatments at different mass con-centrations had strong concentration-dependent protective effects against H2O2-induced oxidative damage in HepG2 cells.Compared with the model group,treatment with high mass concentrations of AIP-2 and AIP-3(50 μg/mL)increased cell survival by 54.35％and 60.26％,SOD viability increased by 35.86％and 38.98％,CAT viability increased by 173.02％and 205.42％,GSH-PX viability increased by 50.24％and 50.78％,and MDA content decreased by 46.13％and 53.87％.Conclusion:Artemisia integrifolia polysaccharide has good antioxidant activity.
Artemisia integrifolia polysaccharideultrasonic-assisted enzymatic extractionisolation and purificationHepG2 cellsantioxidant
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