摘要
实时可视化监测鱼肉新鲜度,对于保障食品质量和消费者安全至关重要.以二甲氨基肉桂醛和异弗尔酮丙二腈为原料,合成一种具有供体(D)-π-受体(A)结构的荧光探针CDI,用来实时监测三文鱼肉的新鲜度.此探针对13种胺类物质响应明显,且对水产品腐败过程中产生的尸胺的荧光检测限低至9.91 µmol/L.采用物理沉降法将探针CDI负载到纤维滤纸上,再加入盐酸使其质子化,得到负载探针CDI+H+的指示标签.将指示标签置于三文鱼肉上空时,随着三文鱼肉新鲜度的下降,指示标签从白色变为浅黄色直至黄色,荧光逐渐开启,从紫粉色变为橙红色.通过对照总挥发性盐基氮值,验证了负载探针CDI+H+的指示标签可以通过比色荧光双响应信号很好地区分三文鱼肉的3个新鲜度等级.
Abstract
Real-time and visual monitoring fish freshness is crucial to safeguard food quality and consumer safety.In this study,a fluorescent probe CDI with a donor(D)-π-acceptor(A)structure was synthesized from 4-(dimethylamino)cin-namaldehyde and isophorone malononitrile to monitor the freshness of salmon in real time.The probe responded apparent-ly to 13 amines and its fluorescence detection limit was as low as 9.91 μmol/L for the cadaverine produced during the spoilage process of aquatic products.The indicator label loaded with probe CDI+H+was obtained by physically depositing probe CDI on fiber filter paper,and then hydrochloric acid was added to protonate it.When the indicator label was placed over salmon meat,the indicator label changed from white to light yellow until yellow,and the fluorescence grad-ually turned on from purplish pink to orange-red as the freshness of salmon meat decreased.By comparing with the total volatile base nitrogen value,it was verified that the indicator label loaded with probe CDI+H+could effectively distinguish the three freshness levels of salmon by colorimetric and fluorescent dual response.
基金项目
国家自然科学基金青年科学基金(32201948)
辽宁省科技厅自然科学研究项目(2022-BS-304)
辽宁省教育厅项目(LJKMZ20221480)
辽宁省教育厅项目(LJKQZ2021135)
维普
万方数据