Objective:To study the chemical constituents of Polygonum multiflorum leaves and lay a foundation for the research and development of functional food of P.multiflorum.Methods:Organic solvent fractions was performed on the alcohol extract of P.multiflorum leaves to obtain different fractions parts such as petroleum ether,dichloromethane,ethyl acetate,n-butanol,and water.Anti-inflammatory activities were carried out on different extraction parts.The fractions with strong anti-inflammatory activity were separated and purified by using silica gel and C18 column combined with semipreparative HPLC methods.Results:The ethyl acetate fraction had no cytotoxicity on RAW264.7 cells in the con-centration range of 0-100 μg/mL and showed dose-dependent inhibition of NO production.Petroleum ether and dichloromethane fractions displayed no cytotoxicity in the concentration range of 0-25 μg/mL,the inhibitory rate on NO of dichloromethane fraction(51.47％)was higher than the fraction of petroleum ether(43.91％).Eleven compounds were isolated from the fractions of ethyl acetate and dichloromethane.The chemical structures were identified as 2,3,4,6-te-trahy-droxyacetophenone-3-O-β-D-glucoside(1),myricitrin(2),quercitrin(3),afzelin(4),β-sitosterol(5),n-hex-acos-5,8,11-trienoic acid(6),hexacosane(7),α-linolenic acid(8),kaempferol-3-O-rutinoside(9),myristic acid(10)and quercetin(11).Conclusion:P.multiflorum leaves showed good anti-inflammatory activity,and compounds 2,4,6,7,8,9,10,11 were isolated and obtained from P.multiflorum for the first time.
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