Protective Effect of EPA/DHA-rich Phosphatidylcholine on SH-SY5Y Cells Injured by MPP+
To investigate the anti-Parkinson's disease(PD)activity of EPA/DHA-rich phosphatidylcholine(PCEPA/DHA)and related mechanisms,MPP+-induced SH-SY5Y cells were used to construct a Parkinson's disease cell model,and the ef-fects of EPA/DHA-phosphatidylcholine(PCEPA/DHA)on the viability,cell morphology and ROS levels of MPP+-induced SH-SY5Y cells were analyzed,and the mechanism was explored by transcriptomic techniques.The results showed that the optimal condition for PD modeling was 600 μmol/L MPP+treatment of SH-SY5Y cells for 24 h.The relative cell viability of the PD model decreased by(53.71±3.21)％.PCEPA/DHA dose-dependently enhanced MPP+-induced SH-SY5Y cell viabili-ty,improved cell morphology and reduced cellular oxidative stress levels.Transcriptomic results showed that the protective effects of PCEPA/DHA on MPP+-induced SH-SY5Y cells were closely associated with improved mitochondrial dysfunction,im-proved dopamine and synuclein alpha-related functions,and inhibition of apoptosis.
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