Objective:This experiment analyzed the resveratrol composition in peanut red coat to investigate the antioxi-dant effects of its main component trans-resveratrol(RES)on human embryonic kidney cell 293(HEK293T)and its po-tential molecular mechanism.Methods:The resveratrol in peanut red coat was extracted by ultrasonic-assisted enzymatic method,and the constituents of the crude extract of resveratrol in peanut red coat were analyzed by high performance liquid chromatography-mass spectrometry(HPLC-MS).The highest toxicity of RES on HEK293T cells was detected by cell viability assay,its effects of Keap1-Nrf2-ARE antioxidant signaling pathway was detected by luciferase reporter gene assay and Western blot assay,and its in vitro antioxidant capacity was determined by DPPH free radical clearance.Re-sults:The results of HPLC-MS showed that resveratrol glycosides,paclitaxel and RES were contained in peanut red coat.Since resveratrol mainly exists in the form of trans-resveratrol in nature,RES was selected for the subsequent experi-ments.The results of cell viability assay showed that the highest non-toxic concentration of RES on HEK293T cells was 50 μmol/L,and the in vitro antioxidant effects was concentration-dependent.Western blot results showed that RES in-duced Nrf2-mediated expression of three target proteins heme oxygenase 1(HO-1),quinone oxidoreductase 1(NQO1)and glutamate cysteine ligase(GCLM).In addition,the results of the DPPH radical scavenging assay showed that RES could effectively scavenge DPPH radicals and had in vitro antioxidant capacity.Conclusion:The major components of resveratrol in peanut red coat have antioxidant effects on HEK293T cells and can activate a potential antioxidant activity through the Keap1-Nrf2-ARE signaling pathway.
维普
万方数据