Study on the Method of Optimizing the Resistance of Lactobacillus acidophilus JYLA-191
In order to improve the stress resistance of probiotics,the core-shell structure of Lactobacillus acidophilus JY-LA-191 dropping pills were prepared by coaxial dropping method with gelatin/pectin/sodium alginate complex as wall ma-terial and oil gel as carrier,the morphology and stability of the strain during processing,storage,heat treatment and di-gestion were evaluated.The results showed that the double-layer dropping pills had obvious core-shell structure and the strains were distributed evenly in the inner oil gel.After processing,the viable count of the double-layer dropping pills decreased by 1.02 lg(CFU/g),which was lower than that of the single-layer dropping pills by 0.89 lg(CFU/g).Both kinds of dropping pills could pass through the upper digestive tract and be released into the colon.The viable bacterial counts of single-layer and double-layer dropping pills could reach 6.38 lg(CFU/g)and 8.43 lg(CFU/g)after continuous digestion.Lactobacillus acidophilus JYLA-191 in double-layer dropping pills exhibited better resistance in high concentra-tions of bile salts than in single-layer dropping pills,with no significant loss of cellular activity(P＞0.05).Both single-layer and double-layer dropping pills increased the heat resistance of Lactobacillus acidophilus JYLA-191,and the activ-ity of double-layer dropping pills could still reach 5.39 lg(CFU/g)after 70℃ heat treatment.The two kinds of dropping pills were stored at 4 ℃ and 25 ℃ to enhance the stability of cells,and the storage effect at 4 ℃ was better than that at single-layer dropping pills and double-layer dropping pills.The results showed that the double-layer dropping pills could effectively enhance the stress resistance of Lactobacillus acidophilus JYLA-191 in harsh environment.
万方数据
维普
