In order to investigate the quorum-sensing inhibition of 2-methyl-2-pentenoic acid and its quorum-sensing ef-fect on Pseudomonas fragi by using the reporter strain Chromobacterium violaceum 026(CV026).The inhibition effect of 2-methyl-2-pentenoic acid on the population induction of P.fragi was analyzed by bacterial apoptosis rate,gas chro-matography-mass spectrometry analysis,biofilm inhibition effect,the amount of extracellular polymer formed,bacterial swarming and swimming.It was demonstrated that the minimum inhibitory concentration of 2-methyl-2-pentenoic acid was 0.5 mg/mL,and 2-methyl-2-pentenoic acid could reduce the ability of CV026 to produce purple bacteriocin at sub-in-hibitory concentration and did not affect the apoptosis.The population-sensing signal molecules C8-HSL and C10-HSL produced by P.fragi were detected quantitatively by gas chromatography-mass spectrometry,and 2-methyl-2-pentenoic acid had an inhibitory effect on the secretion of the produced signal molecules.The biofilm formation of P.fragi by subinhibitory concentration 2-methyl-2-pentenoic acid was 21.06％,and the inhibition of bacterial swarming and swim-ming was reduced by 34.06％and 75.02％,respectively,in a concentration-dependent manner.In conclusion,2-methyl-2-pentenoic acid had good population induction inhibitory property and could be used as a population induction inhibitor in aquatic preservation.
维普
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