Antibacterial Activity of Salted Penaeus vannamei Antimicrobial Peptide PV-M7 against Vibrio parahaemolyticus
Antimicrobial peptides are novel biological preservatives in the food industry,and salted fermented foods are one of the sources of antimicrobial peptides(AMPs).In this study,ultra-performance liquid chromatography-mass spec-trometry(UPLC-MS)was used to identify small molecule peptide sequences from salted Penaeus vannamei,and potential antimicrobial peptides were analyzed and screened by bioinformatics.Antibacterial activity and mechanism of AMPs a-gainst Vibrio parahaemolyticus were evaluated by minimum inhibitory concentration,time-kill curve,cell membrane per-meability,and circular dichroism.The results showed that the minimum inhibitory concentration of the novel AMP(PV-M7)from salted Penaeus vannamei was 15.6 μg/mL against Vibrio parahaemolyticus,and the antibacterial activity was exerted within 1 h.After PV-M7 acted on Vibrio parahaemolyticus,it could increase the permeability of the cell mem-brane,leak the macromolecular substances(nucleic acid,protein)in the cell,at the same time,the surface of the cell membrane was blurred,and the density of the cytoplasm decreased.When PV-M7 contacts the cell membrane,its sec-ondary structure changed from random coil to α-helical,this structural transformation was the key mechanism of its an-tibacterial activity.
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