Genome Sequencing Analysis and Gene Function Annotations of Functional Brewing Fungi
In order to explore the metabolic mechanism of Pichia kudriavzevii FJZ in the brewing process of light-aroma Baijiu,the genome of the strain FJZ was sequenced based on Illumina Novaseq and PacBio sequencing platform,and the sequencing data was compared and analyzed in the carbohydrate active enzyme(CAZy)database,gene function an-notation was also performed in GO,KOG and KEGG databases.In the CAZy analysis,it was shown that the strain FJZ had the activity of vanillyl alcohol oxidase(VAO)that catalyzed the conversion of phenolic compounds into aromatic compounds,carboxylesterase catalyzed the synthesis and decomposition of ethyl acetate and ethyl lactate,it had α-1,2-mannose transferase to catalyze mannose to form a precursor to polyterpenoid oligosaccharides.In the GO database,6 320 genes with molecular function was annotated,it included transferase activities related to ester synthesis,they had the function of transferring alkyl or aryl groups,sugar groups,acyl groups,etc.In the KEGG's seven functional database,a total of 7 815 genes were annotated,of which 1 406 genes were metabolic-related genes,and 30 genes were included terpenoids and polyketides.KOG annotated a total of 3 704 genes,coenzyme transport and metabolism 83 genes.This study provides a basis for the study of enzyme lines in the brewing process of light-aroma baijiu,as well as the formation mechanism of liquor flavor substances and the structure of functional bacteria of liquor.
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