To investigate the mechanism of the hypoglycemic effect of immature guava fruit extract on diabetic mice,a diabetes mouse model was established through streptozotocin induction.The mice were divided into normal,normal hypo-glycemic,model,positive control,and high,medium,and low dose groups of immature guava fruit extract.The fasting blood glucose,glucose tolerance,and lipid profile of the mice were measured.Pathological observations were conducted on the liver,kidney,and pancreatic tissues,and a non-targeted metabolomics study was carried out on the serum sam-ples to investigate the therapeutic effects of immature guava fruit on diabetic mice.The results showed that,compared to the model group,the high-dose immature guava fruit extract significantly reduced the blood glucose levels(P＜0.05)and improved the glucose tolerance(P＜0.05)in diabetic mice.It also lowered the levels of serum triglycerides and total cholesterol(P＜0.05),and exhibited a certain protective effect on the liver,kidney,and pancreatic tissues(tissue mor-phology integrity)of the diabetic mice.Through serum metabolomics analysis,27 significantly altered differential metabo-lites had been screened out,and it had been clarified that cortisol synthesis and secretion were the most relevant metabolic pathways.Conclusion:The aqueous extract of immature guava fruit had exhibited certain effects in lowering blood glucose,regulating lipid levels,and protecting liver,kidney,and pancreatic tissues.It had achieved the improve-ment of diabetes by regulating the body's cortisol hormone levels.
NETL
NSTL
万方数据
