Zeaxanthin Alleviates Chronic Inflammation of 3T3-L1 Adipocytes Induced by Lipopolysaccharide
Objective:To explore the anti-inflammatory mechanism of zeaxanthin(ZEA,a natural carotenoid)based on the NF-KB signaling pathway in lipopolysaccharide-induced(LPS-induced)3T3-L1 adipocytes chronic inflammatory.Methods:The inflammatory models were established by using LPS to stimulate mature 3T3-L1 adipocytes and were inter-vened with different concentrations of ZEA(0,5,10,15 μmol/L).The content of inflammatory-related cytokines was measured,and their mRNA expression levels were detected by Quantitative Real-time PCR(qPCR).In addition,the mRNA and protein expression levels of signaling pathway cytokines were detected by Western blot.Results:Compared with the normal group,ZEA intervention significantly down-regulated the secretion of pro-inflammatory cytokines IL-1β,IL-6,TNF-α and MCP-1(P＜0.001)while up-regulated the content of anti-inflammatory cytokines IL-10 and ADPN(P＜0.001)in the inflammatory models.Moreover,qPCR indicated that ZEA inhibited the mRNA expression of pro-in-flammatory cytokines(P＜0.001)while recovering the mRNA expression of anti-inflammatory cytokines(P＜0.001).Fur-thermore,Western blot analysis suggested that ZEA notably decreased the protein and phosphorylation expression of catalytic subunits of IκB kinases(IKKα and IKKβ)(P＜0.001),then increased the combination of inhibitory protein IKBα and nuclear transcription factor NF-κB p65,and eventually inhibited the transcription of NF-κB p65 in the nucle-us.Conclusions:Zeaxanthin effectively alleviates obesity-caused chronic inflammation by inhibiting NF-κB signaling path-way activation and regulating inflammatory cytokines secretion.
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