玉米黄素减轻脂多糖诱导的3T3-L1脂肪细胞慢性炎症
Zeaxanthin Alleviates Chronic Inflammation of 3T3-L1 Adipocytes Induced by Lipopolysaccharide
赵有伟 1吴熙 1朱晨 1刘美宏 1郑明珠 1蔡丹 1刘回民 1刘景圣1
作者信息
- 1. 吉林农业大学食品科学与工程学院 小麦和玉米深加工国家工程研究中心 长春 130118
- 折叠
摘要
目的:探究一种天然类胡萝卜素——玉米黄素(ZEA),基于NF-κB信号通路减轻脂多糖(LPS)诱导的3T3-L1脂肪细胞慢性炎症并发挥抗炎的作用机制.方法:以LPS刺激成熟的3T3-L1脂肪细胞建立炎症模型,用不同浓度的ZEA(0,5,10,15 μmol/L)进行干预,检测脂肪细胞炎症相关因子的含量,并通过实时荧光定量PCR(qPCR)检测其mRNA表达水平,用蛋白免疫印迹法检测信号通路因子的蛋白表达水平.结果:与LPS组相比,ZEA干预显著抑制炎症细胞模型中促炎因子IL-1β、IL-6、TNF-α和MCP-1分泌(P＜0.001),同时促进抗炎因子IL-10和ADPN产生(P＜0.001).此外,qPCR结果显示ZEA能有效抑制促炎因子,同时恢复抗炎因子的mRNA表达水平(P＜0.001).蛋白免疫印迹结果表明,ZEA能明显降低IκB激酶催化亚基IKKα和IKKβ的蛋白和磷酸化表达(P＜0.001),增强抑制蛋白IκBα与核转录因子NF-κB p65的结合,最终抑制NF-κB p65进行核内转录.结论:ZEA可通过抑制NF-κB信号通路激活及调控炎性因子的分泌,改善肥胖引起的慢性炎症.
Abstract
Objective:To explore the anti-inflammatory mechanism of zeaxanthin(ZEA,a natural carotenoid)based on the NF-KB signaling pathway in lipopolysaccharide-induced(LPS-induced)3T3-L1 adipocytes chronic inflammatory.Methods:The inflammatory models were established by using LPS to stimulate mature 3T3-L1 adipocytes and were inter-vened with different concentrations of ZEA(0,5,10,15 μmol/L).The content of inflammatory-related cytokines was measured,and their mRNA expression levels were detected by Quantitative Real-time PCR(qPCR).In addition,the mRNA and protein expression levels of signaling pathway cytokines were detected by Western blot.Results:Compared with the normal group,ZEA intervention significantly down-regulated the secretion of pro-inflammatory cytokines IL-1β,IL-6,TNF-α and MCP-1(P＜0.001)while up-regulated the content of anti-inflammatory cytokines IL-10 and ADPN(P＜0.001)in the inflammatory models.Moreover,qPCR indicated that ZEA inhibited the mRNA expression of pro-in-flammatory cytokines(P＜0.001)while recovering the mRNA expression of anti-inflammatory cytokines(P＜0.001).Fur-thermore,Western blot analysis suggested that ZEA notably decreased the protein and phosphorylation expression of catalytic subunits of IκB kinases(IKKα and IKKβ)(P＜0.001),then increased the combination of inhibitory protein IKBα and nuclear transcription factor NF-κB p65,and eventually inhibited the transcription of NF-κB p65 in the nucle-us.Conclusions:Zeaxanthin effectively alleviates obesity-caused chronic inflammation by inhibiting NF-κB signaling path-way activation and regulating inflammatory cytokines secretion.
关键词
玉米黄素/脂多糖/慢性炎症/NF-κB信号通路/抗炎作用Key words
zeaxanthin(ZEA)/lipopolysaccharide(LPS)/chronic inflammation/NF-κB signaling pathway/anti-inflam-matory effect引用本文复制引用
基金项目
吉林省自然科学基金项目(20200201148JC)
吉林省中青年科技创新创业卓越人才(团队)项目(创新类)(20210509026RQ)
出版年
2024
NETL
NSTL
万方数据