东北传统大酱中产抗菌物质菌株的筛选及安全性研究
Screening and Safety Study of Antimicrobial Substance Producing Strains in Traditional Soybean Paste in Northeast China
任晓蕾 1鲍捷 1郭宝松 1董亮 1纪超凡 1张素芳1
作者信息
- 1. 大连工业大学食品学院 海洋食品加工与安全控制全国重点实验室 国家海洋食品工程技术研究中心海洋食品精深加工关键技术省部共建协同创新中心 辽宁大连 116034
- 折叠
摘要
以东北大酱为原料,分离、鉴定产抗菌物质的微生物菌株,分析其抗菌能力.结果显示,共分离得到44株具有抑菌活性的菌株,主要为芽孢杆菌属.通过抑菌谱试验发现,贝莱斯芽孢杆菌R2可抑制6种食源性致病菌生长,具有抑菌广谱性,并可从中扩增出其编码非核糖体脂肽类物质杆菌霉素D、丰原素、鞭毛蛋白、伊枯草菌素A、芽孢菌溶素、枯草菌脂肽和蛋白物质类TasA的相关基因片段.通过对贝莱斯芽孢杆菌R2的功能、表型和安全相关特性的研究,发现菌株贝莱斯芽孢杆菌R2菌株不溶血,对10种抗生素敏感,并具有蛋白酶、淀粉酶、纤维素酶和果胶酶活性.本研究为酱类低盐发酵微生物资源的高值化利用提供参考.
Abstract
The objective of this study was to isolate and identify microorganisms capable of producing antimicrobial com-pounds from Northeast China soybean paste and to analyse their antimicrobial capacity.The results showed that a total of 44 strains with antibacterial activity were isolated,mainly Bacillus spp.Of these,Bacillus velezensis R2 was identified as capable of inhibiting the growth of six foodborne pathogenic bacteria,with a broad spectrum of bacterial inhibition.Additionally,non-ribosomal lipopeptides encoding bacillomycin D,fengycin,flagellin,iturin A,bacilysin,surfactin,and the protein class TasA were amplified from B.velezensis R2.The functional,phenotypic and safety-related characteriza-tion of B.velezensis R2 revealed that it was non-hemolytic,sensitive to 10 antibiotics,and possessed protease,amy-lase,cellulase,and pectinase activities.This study aims to provide a reference for the high-value utilization of low-salt fermentation microbial resources of sauces.
关键词
东北大酱/抑菌活性菌株/芽孢杆菌/抗菌物质/安全性Key words
northeast soybean paste/antibacterial microorganism/Bacillus/antibacterial substance/safety引用本文复制引用
基金项目
国家自然科学基金面上项目(32072185)
出版年
2024
NETL
NSTL
万方数据