In this research,the effects of heating treatment conditions on LOX activity and conformation were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),circular dichroism spectroscopy,surface hy-drophobicity,fluorescence spectroscopy and molecular docking.The results showed that 120 ℃ 80 s+140 ℃ 15 s and 100 ℃ 10 min heating treatment could inhibit 67.47％and 60.53％of LOX activity.SDS-PAGE results showed that the molecular mass of the enzyme did not change significantly with heating conditions,but the band strength decreased.Moreover,heating treatment caused the decrease in α-helix and β-sheet,and increased in fluorescence intensity and surface hydrophobicity,suggesting that the greatly changed secondary and tertiary structures of LOX lead to a rapid de-cline in LOX activity.Molecular docking determined that the microenvironment surrounding the non heme iron at the ac-tive center of LOX was susceptible to temperature changes.Therefore,it was speculated that high-temperature heating treatment may affect the LOX activity by destroying the non-covalent interaction force that maintains the structure of en-zyme.High-temperature short-time elevated-temperature inactivation of LOX is an appropriate heating method.The find-ings could provide a theoretical reference for the widespread application of LOX activity control in soybean processing.
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