摘要
以金线鱼肌球蛋白为原料,添加10％不同复配比例的马铃薯淀粉(PS)-卵清蛋白(OVA),研究其与肌球蛋白间的相互作用及其对肌球蛋白凝胶特性的影响.采用粒径、Zeta电位、疏基、表面疏水性和紫外光谱分析PS-OVA-肌球蛋白混合体系中组分的相互作用,进一步结合流变行为、分子间作用力、微观结构等揭示PS-OVA-肌球蛋白互作对肌球蛋白凝胶的影响.结果表明,添加10％的PS-OVA复配物后,PS-OVA-肌球蛋白混合体系的紫外吸光度、总疏基含量和表面疏水性分别增加了5.53％,24.28％和17.53％,净电荷减小40.75％,表明PS-OVA-肌球蛋白混合体系中存在疏水作用、氢键、二硫键等非共价作用和共价作用,促使肌球蛋白构象变化.同时,随着外源复配物中PS的增加和OVA的减少,PS-OVA-肌球蛋白混合凝胶的凝胶强度、硬度、β-折叠含量和储能模量分别先增加了 7.68％,20.10％,11.03％和12.07％后又下降至0.94％,7.77％,0.08％和4.99％.当PS-OVA复配比为3∶7时,混合体系的紫外吸光度最高,净电荷最小,粒径、疏基含量和表面疏水性最大;加热后3∶7的PS-OVA组混合凝胶的凝胶强度、硬度和β-折叠含量最高,且凝胶网络结构最致密、均匀.结论:质量比(3∶7)的PS-OVA复配可促进混合体系中组分的相互作用,通过疏水作用、氢键、二硫键等促进蛋白分子的交联聚集,填充肌球蛋白凝胶网络,从而改善肌球蛋白的凝胶特性.
Abstract
In this study,myosin isolated from Nemipterus virgatus was added with 10％potato starch(PS)-ovalbumin(OVA)complex with different ratios to study the interactions between PS-OVA and myosin as well as their effects on the gel properties of myosin.The component interactions in the PS-OVA-myosin composite system were determined by particle size,zeta potential,sulfhydryl group,surface hydrophobicity and UV spectra,and the effect of PS-OVA-myosin interactions on myosin gel was further disclosed by rheological behavior,intermolecular forces,microstructure,etc.The results showed that after adding 10％PS-OVA complex,the UV absorbance,total sulfhydryl content and surface hy-drophobicity of the PS-OVA-myosin composite system increased by 5.53％,24.28％and 17.53％,respectively,and the net charge decreased by 40.75％,indicating that there were non-covalent and covalent interactions such as hydrophobic interaction,hydrogen bond,and disulfide bond in PS-OVA-myosin composite system,which promoted the conformational change of myosin.Simultaneously,with the increase of PS and decrease of OVA in the exogenous compound,the gel strength,hardness,-sheet content and storage modulus of the PS-OVA-myosin composite gel increased by 7.68％,20.10％,11.03％and 12.07％,respectively,and then decreased to 0.94％,7.77％,0.08％and 4.99％.When the ratio of PS-OVA was 3:7,the composite system had the highest UV absorbance,the smallest net charge,and the largest particle size,sulfhydryl content,and surface hydrophobicity.The gel strength,hardness,and β-sheet content of the composite gel were the highest after heating when the ratio of PS-OVA was 3:7,and the gel network was most compact and homogeneous.Conclusion:The PS-OVA complex with mass ratio of 3:7 could promote the component interaction of the PS-OVA-myosin composite system,facilitate the aggregation and cross-linking of myosin through the interaction forces such as hydrophobic interactions,hydrogen bonds,disulfide bonds,etc.,fill the myosin gel network,which fur-ther improve the gel properties of myosin.
基金项目
国家自然科学基金区域创新发展联合基金重点支持项目(U20A2067)
NETL
NSTL
万方数据