Protective Effect and Mechanism of Aqueous Extract of Thamnolia vermicularis Ach on Ethanol-Induced Acute Alcoholic Liver Injury in Mice
Objective:The purpose of the present study was to investigate the protective effect of aqueous extract of Thamnolia vermicularis Ach(TWA)on acute alcoholic liver injury(AALI)and its mechanism.Methods:The AALI model was induced by gavage of 56％alcohol at a dose of 12 mL/kg of the experiment.Its mechanisms were evaluated by weight loss,liver index,liver function,ability to metabolize alcohol,oxidative stress levels,inflammatory factors,changes in key protein expression and the effects on intestinal flora.Results:TWA significantly reduced the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),increased the level of ethanol dehydrogenase(ADH),improved liver function and accelerated ethanol metabolism in mice.TWA also increased the activities of super-oxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in serum and liver,decreased malondialdehyde(MDA)content,and reduced level of oxidative stress in the mouse caused by ethanol.In addition,TWA reduced the inflamma-tory response of the body by suppressing tumour necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in the liver and serum.TWA enhanced Nrf2 pathway function and inhibited NF-κB pathway activation,thereby inhibiting liver in-flammation and improving the antioxidant stress effect in mice with acute alcoholic liver injury.Meanwhile,the expres-sion of CYP2E1,a key protein of alcohol metabolism,was down-regulated to accelerate hepatic alcohol metabolism and reduce hepatic alcohol metabolic injury.TWA promoted the proliferation of Lactobacillus and Bifidobacterium,and re-duced the colony numbers of Enterococcus,E.coli and Peptococcus,so improved the intestinal environment.Conclusion:TWA could improve liver function and alcohol metabolism,enhance antioxidant capacity,reduce the level of inflammato-ry factors,inhibit the expression of key proteins of Nrf2/NF-κB pathway and maintain intestinal homeostasis,so improve AALI in mice.
aqueous extract of Thamnolia vermicularis Achliver injuryoxidative stressinflammationalcohol
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