The Antioxidant Mechanism of Lactiplantibacillus plantarum CY1-2 Based on HeLa Cell Model
In the study,a model of HeLa cells protected by Lactiplantibacillus plantarum CY1-2 against 2,2'-azobis[2-methylpropiona midine]dihydrochloride(ABAP)damage was established.The antioxidant mechanism of L.plantarum CY1-2 was investigated using cell proliferation test,morphological observation and western blot analysis.The results showed that the cell protective model conditions were as follows:108 CFU/mL of L.plantarum CY1-2 protected cells for 3 h,and then 110 mmol/L of ABAP damaged cells for 3 h.Under this model condition,both the cell and the cell-free extract of L.plantarum CY1-2 could effectively improve the viability of HeLa cells,and the cell survival rate increased by 9.41％ and 25.71％ ,respectively.Meanwhile,microscopic observation showed that compared with the injured group,HeLa cells protected by the cell-free extract of L.plantarum CY1-2 were large and plump,with clear edges and good extensiability.Western blot analysis indicated that,compared with the injured group,the cell-free extract of L.plantarum CY1-2 could positively regulate the expression of kelch-like ECH-associated protein 1(Keap1),nuclear factor erythroid 2-related factor(Nrf2)and heme oxygenase-1(HO-1)proteins in HeLa cells,resulting in increasing by 18％ ,47％ and 58％ respectively.L.plantarum CY1-2 protects HeLa cells from oxidative damage by activating Keap1-Nrf2-ARE signaling pathway to improve its antioxidant capacity.This study was expected to provide a theoretical basis for the de-velopment of lactic acid bacteria antioxidants.
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