The Antioxidant Activities and Cell Protective Effects of Different Polar Samples of Polygonatum kingianum
Polygonatum kingianum,belong to the genus Polygonatum(Liliaceae),mainly distributed in subtropical in the southwest of China,including Guizhou,Sichuan,and Yunnan.The dried rhizomes of P.kingianum have been used as a famous homologous plant of medicine and food for thousands of years.The paper was investigated the antioxidant activi-ties and cell protective effects of Polygonatum kingianum.Using D101 macroporous resin,the methanol extract was eluted by different volume fraction of methanol water(20％ ,40％ ,60％ ,80％ ,100％ )to obtain the different polar samples.The contents of total phenolic(TPC)and total flavonoids(TFC)in different polar samples were determined.The antioxi-dant activities were evaluated by 2,2-diphenyl-1-picryhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sul-fonic acid)(ABTS)methods.Furthermore,the cytoprotective effects of different polar samples were performed on hydro-gen peroxide(H2O2)-induced oxidative stress in HepG2 cells.The intracellular ROS production,the content of lipid peroxides(MDA)and the activity of enzymes(superoxide dismutase(SOD),glutathione peroxidase(GSH-px))were measured.Principal component analysis(PCA)was used to analyze the correlations among the TPC and TFC,antioxidant activities(DPPH and ABTS),the contents of intracellular ROS and MDA,and the activities of intracellular oxidase(SOD,GSH-px).The results showed that the antioxidant capacities of different polar samples were positively correlated with TPC and TFC.The antioxidant capacity of the sample increased with the increasing of total phenolic and flavonoids content.The TPC of the 40％ and 60％ methanol samples were(40.27±0.59)mg GAE/g extract and(51.34±2.08)mg GAE/g extract,respectively.While TFC were(23.95±1.73)mg RE/g extract and(24.97±2.75)mg RE/g extract,respec-tively.The 40％ and 60％ methanol samples exhibited the highest phenolic and flavonoid contents,and displayed signifi-cantly free radical scavenging activities in vitro.Additionally,these samples markedly inhibited intracellular ROS produc-tion,enhanced SOD and GSH-px levels,and reduced MDA content.In conclusion,different polar samples of P.kingianum had certain antioxidant activities and cell protective effects,and 40％ and 60％ methanol samples displayed the best antioxidant activities and cell protective effects.
万方数据
维普
