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杨梅花色苷体外降糖活性研究

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为了评价杨梅果实花色苷提取物(AECBF)的体外降糖活性,研究AECBF体外对α-淀粉酶和α-葡萄糖苷酶的抑制作用及其动力学,以及对人肝癌HepG2细胞葡萄糖摄入和消耗的影响.结果表明:AECBF对α-淀粉酶和α-葡萄糖苷酶均具有较强的抑制作用,其IC50值分别为22.54 µg/mL和3.51 µg/mL,酶促抑制动力学结果显示:其对2种酶的抑制类型均为混合型,对于α-淀粉酶,竞争性抑制占主体,而对于α-葡萄糖苷酶,反竞争性抑制是主要的.此外,0.1~10 µg/mL的AECBF作用HepG2细胞24 h,未显示出明显细胞毒性(P>0.05),而1~5 µg/mL的AECBF作用24 h可显著增加HepG2细胞葡萄糖摄入和消耗(P<0.05),且与未干预组比较,HepG2细胞葡萄糖摄入水平和葡萄糖消耗量分别在AECBF质量浓度为2 µg/mL和5μg/mL时最高,分别增加了 72%和44%.这些研究结果说明杨梅花色苷体外具有显著的降糖活性.
Studies on Hypoglycemic Activities of Chinese Bayberry Anthocyanins in Vitro
Inhibition and kinetics of α-amylase and α-glucosidase,as well as glucose uptake and consumption of human hepatoma HepG2 cell were investigated to assess the in vitro glucose-lowering effect of anthocyanin extract of Chinese bayberry(Myrica rubra Sieb.et Zucc.)fruit(AECBF).The results showed that AECBF exhibited potently inhibitory ef-fect on α-amylase and α-glucosidase,with IC50 value of 22.54 μg/mL and 3.51 μg/mL,respectively.The results of enzy-matic inhibition kinetics indicated that both α-amylase and α-glucosidase gave a mixed-type inhibition,and competitive inhibition was predominant in α-amylase while uncompetitive inhibition in α-glucosidase.In addition,no obvious cyto-toxicity of HepG2 was observed after treated with AECBF for 24 h,ranged from 0.1 μg/mL to 10 μg/mL(P>0.05),and glucose uptake and consumption of HepG2 cells were increased notably after treatment with 1 μg/mL to 5 μg/mL for 24 h(P<0.05).Compared to normal control,AECBF-treated HepG2 cells gained the maximum glucose uptake and glucose consumption at 2 μg/mL and 5 μg/mL,respectively,corresponding to an increase by 72% and 44% ,respectively.These results indicated that Chinese bayberry anthocyanin exhibits potent in vitro hypoglycemic activities.

bayberry fruitanthocyaninsstarch-digesting enzymesinhibition kineticsglucose uptake and consumption

章鸿彬、刘合生、陈杨、蔡将波、曹少谦、戚向阳

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浙江万里学院 生物与环境学院 浙江宁波 315100

杨梅果实 花色苷 淀粉消化酶 抑制动力学 葡萄糖摄入和消耗

2024

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2024.24(8)