Effects of Cryoprotectant to Freeze-dried Survival Rate of Lactobacillus fermentum BLHN3
In order to improve the freeze-dried survival rate of Lactobacillus fermentum BLHN3,the effect of cryoprotec-tant on the freeze-dried resistance of Lactobacillus fermentum BLHN3 and the storage stability of freeze-dried Lactobacil-lus powder were investigated.The experimental results showed that the survival rate of Lactobacillus fermentum BLHN3 was the highest with 3％ L-glutamate+5％ maltose+5％ skim milk,and the number of viable count reached 1.48×1011 CFU/g.Scanning electron microscopy showed that cryoprotectants could maintain the integrity of the cell membrane of Lactobacillus after freeze-dried.The FTIR results showed that the cryoprotectant could reduce the damage of freeze-dried cells by binding with the cells.The activities of lactate dehydrogenase(LDH)and ATPase were significantly increased,but the pyruvate kinase(PK)activity was not significantly different after freeze-dried.In addition,the accelerated storage test showed that low temperature storage was an effective method for the preservation of powder.Conclusion:Lactobacil-lus fermentum BLHN3 could be prepared into a high activity freeze-dried starter with 3％ L-glutamate sodium,5％ mal-tose,5％ skim milk as cryoprotectants.
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