Pseudomonas fluorescens is a dominant spoilage bacterium commonly found in fresh aquatic products,and its spoilage ability is regulated by a quorum-sensing system of N-homoserine lactones(AHLs).Based on the quorum-sens-ing quenching activity,the type of quenching activity substance from strain YF-2 was analyzed using heat inactivation and acid sensitivity tests.The results showed that strain YF-2 had a significant quenching effect on P.fluorescens AHLs,with a degradation rate of 100％ .Its quenching active substance existed in the crude cell extract(CCE),which was preliminarily identified as acyltransferase or oxidoreductase by heat inactivation and acid sensitivity assays.The degrada-tion of C4-HSL,C6-HSL and C8-HSL treated by the CCE at protein concentrations of 20,40 μg/mL and 80 μg/mL ranged from 52.8％ to 100％ ,and the inhibition and clearance rate of P.fluorescens biofilms ranged from 25.84％ to 56.63％ and 26.12％ to 59.35％ ,respectively.In addition,the confocal laser scanning microscopy showed that the CCE reduced the thickness of P.fluorescens biofilm and disrupted the biofilm structure.Meanwhile,Bacillus cereus was recog-nized as the source of strain YF-2.The application of B.cereus to control the spoilage bacterium P.flluorescens in aquatic goods will have a theoretical foundation thanks to this study.
关键词
群体感应/蜡样芽孢杆菌/荧光假单胞菌/群体感应淬灭酶
Key words
quorum sensing/Bacillus cereus/Pseudomonas fluorescens/quorum-quenching enzyme
维普
万方数据