中国食品学报2024,Vol.24Issue(9) :31-39.DOI:10.16429/j.1009-7848.2024.09.003

漆酶-Ca2+/Mg2+双诱导乳液凝胶的构建及油-水双相负载功能因子的应用

Preparation of Laccase-Ca2+/Mg2+Duo-induced Emulsion Gels and Application of Load Functional Factors in both Oil and Water Phase

戴澍涵 高崇 殷丽君 张明皓
中国食品学报2024,Vol.24Issue(9) :31-39.DOI:10.16429/j.1009-7848.2024.09.003

漆酶-Ca2+/Mg2+双诱导乳液凝胶的构建及油-水双相负载功能因子的应用

Preparation of Laccase-Ca2+/Mg2+Duo-induced Emulsion Gels and Application of Load Functional Factors in both Oil and Water Phase

戴澍涵 1高崇 1殷丽君 1张明皓1
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作者信息

  • 1. 中国农业大学食品科学与营养工程学院 北京 100083
  • 折叠

摘要

为进一步优化乳液凝胶的制备工艺,本研究利用漆酶与钙/镁离子双诱导的方式构建大豆分离蛋白-甜菜果胶乳液凝胶,根据归一化的方法得到凝胶质构特性综合值的计算模型,并提出一种利用较低浓度镁离子替换高浓度钙离子的方案,以此降低凝胶制备过程中离子的添加量.结果表明,对比漆酶与钙离子双诱导乳液凝胶,漆酶与镁离子双诱导乳液凝胶具有更短的凝胶时间(340 s)和相似的流变特性,更均匀的微观结构及油滴分布,更佳的贮藏稳定及在小肠中更高的释放率(β-胡萝卜素释放率73.1%,核黄素释放率85.7%).本研究构建了一种新型的乳液凝胶体系并应用于油-水双相负载递送功能因子领域,同时验证了利用较低浓度镁离子诱导成胶的可行性.

Abstract

In order to optimize the preparation process of emulsion gels,a soy protein isolate-sugar beet pectin emul-sion gel was prepared by duo induction of laccase and Ca2+/Mg2+,and a model for the comprehensive property index of the emulsion gel textural properties was established based on the normalization method.High concentration of Ca2+was replaced with lower concentration of Mg2+to reduce the amount of ions added during the emulsion gel preparation.The results showed that compared with the laccase and Ca2+duo-induced emulsion gel,the laccase and Mg2+duo-induced emulsion gel had shorter gelation time(340 s),similar rheological properties,more uniform microstructure and oil droplet distribution,better storage stability and higher release rate in the small intestine(73.1%for β-carotene,85.7%for ri-boflavin).This study not only prepared a novel emulsion gel that can be used as the multi-phased nutrient carrier,but also offered a possible solution of replacing high concentrations of Ca2+with lower concentrations of Mg2+.

关键词

漆酶-钙/镁离子双诱导/乳液凝胶/大豆分离蛋白/甜菜果胶

Key words

laccase and Ca2+/Mg2+duo induction/emulsion gel/soy protein isolate/sugar beet pectin

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基金项目

山东省重点研发计划项目(2022CXGC010602-02)

国家自然科学基金面上项目(32272337)

出版年

2024
中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCDCSCD北大核心EI
影响因子:1.079
ISSN:1009-7848
参考文献量4
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