Lactic acid bacteria play an essential role in fermentation due to their characteristics and secretion of various metabolites.In this study,two lactic acid bacteria with high β-glucosidase activities were screened from kimchi and i-dentified as Lactobacillus plantarum PC7-C6 and PC7-C11).The probiotic characteristics of the two strains and the en-zymatic characteristics of PC7-C11 which has higher enzyme production ability,were analyzed.The results showed that the two strains were γ-hemolysis,sensitive to ampicillin and clarithromycin,moderately sensitive to tetracycline,ery-thromycin,and clindamycin,and could grew at pH 4.The auto-aggregation rate of the two isolates ranged from 50％to 64％,and the co-aggregation rates with Escherichia coli were more than 60％after co-incubating for 8 hours.The hy-drophobicity of the isolates towards ethyl acetate was 70.41％and 61.19％,and the hydrophobicity of the isolates towards xylene and n-hexane was between 30％and 40％.The two isolates could inhibit the growth Escherichia coli,Haffnia alvei,Staphylococcus saprophytic us,Serratia marcescens,and Staphylococcus equorum.The antioxidant capacity of PC7-C11 was superior to that of PC7-C6,with a 59.33％scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 53.49％scavenging rate of hydroxyl radicals respectively.The enzymatic characteristics of PC7-C11 showed that the opti-mal reaction temperature and pH for enzyme activity were 40 ℃ and pH 7.Ethanol,glucose,dimethyl sulfoxide,SDS,EDTA,Fe2+,Mg2+,Na+,and Ca2+inhibited enzyme activity to varying degrees,while K+has a certain promoting effect on enzyme activity.
维普
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