To improve the bioavailability of buckwheat protein,buckwheat antioxidant peptides(BAPs)was isolated from the fermentation hydrolysate of buckwheat by using Sephadex G-25 and reversed phase-high performance liquid chro-matography(RP-HPLC),and its structure and composition were preliminarily analyzed.The H2O2-induced RAW 264.7 macrophages cell model of oxidative stress was further established to evaluate the antioxidant activity of BAPs.The re sults showed that the BAPs obtained after separation by RP-HPLC has a purity of 96.25％and a molecular weight of 5.8 ku.Vitamin C equivalent antioxidant capacity(VCEAC)was used to indicate that DPPH·,ABTS+·,·OH scavenging ability and reducing power of BAPs were(12.12±0.27),(153.82±5.04),(901.95±39.30),(109.23±1.18)μg/mg,re-spectively.In the RAW 264.7 macrophage oxidative stress model,BAPs with the concentration of 200 µg/mL had a sig-nificant(P＜0.05)effect on all measured antioxidant indicators compared with the model group,which significantly(P＜0.05)promoted the viability of oxidatively damaged cells by 13.80％,reduced the levels of intracellular reactive oxygen species(ROS)by 40.63％and malondialdehyde(MDA)by 11.92nmol/mg prog.Meanwhile,the activities of superoxide dismutase(SOD)and catalase(CAT)were increased by 23.48 and 15.71 U/mg prot at this concentration,respectively.The above results showed that BAPs can effectively inhibit the oxidative stress response of RAW 264.7 macrophages,which could provide a theoretical basis for the development of buckwheat peptides as natural antioxidants.
关键词
荞麦多肽/分离纯化/结构表征/RAW/264.7巨噬细胞/抗氧化
Key words
buckwheat peptide/separation and purification/structure characterization/RAW 264.7 macrophages/antioxi-dant
维普
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