Protective Effect of Pachymic Acid on Okadaic Acid-induced Neurotoxicity in PC12 Cells
Objective:To investigated the protective effects of pachymic acid on okadaic acid(OA)-induced neurotoxicity in PC12 cells and identified a potential mechanism.Methods:Pachymic acid(10,15,20 μg/mL)was used as protective drugs to protect PC12 cells injured by OA(40nmol/L).Cell viability,LDH released,intracellular Ca2+concentration and caspase-3 activity were detected.The levels of bax and p-taus202 were detected by western blotting.Results:Compared with the control group,PC12 cells treated with 40 nmol/L OA was significantly decreased the viability to(64±3.6)％(P＜0.01),increased the release of LDH to(141.3±3.1)％increased intracellular Ca2+concentration to(123.5±5.2)％and caspase-3 activity to(257.0±22.8)％(P＜0.01);compared with the model group,50 μg/mL pachymic acid significantly increased cell viability to(90.5±3.3)％(P＜0.01),decreased LDH released to(117.5±3.8)％,decreased[Ca2+]i,to(123.5±5.2)％and reduced caspase-3 activity to(120.0±4.1)％(P＜0.01).Western blot results confirmed that OA in-creased the protein expression of bax and p-taus202,pachymic acid down-regulated the expression of bax and p-taus202.Conclusion:the protective effect of pachymic acid against OA-induced apoptosis in PC 12 cells was related to the inhibition of tau hyperphosphorylation.
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